4GLK
Structure and activity of AbiQ, a lactococcal anti-phage endoribonuclease belonging to the type-III toxin-antitoxin system
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-10-14 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.140, 54.510, 64.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.670 - 2.160 |
| R-factor | 0.1958 |
| Rwork | 0.194 |
| R-free | 0.22320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xdd |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.180 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.9.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.220 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.090 | 0.600 |
| Number of reflections | 9880 | |
| <I/σ(I)> | 17.1 | 3.7 |
| Completeness [%] | 99.7 | 99.6 |
| Redundancy | 13.7 | 13.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | buffer: 1 M potassium/sodium tartrate, 0.1 M MES, pH 6.0, drop: 200 nL 4.6 mg/mL protein + 100 nL buffer, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
