4GLH
DNA dodecamer containing 5-hydroxymethyl cytosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-09 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.00067 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.637, 41.767, 63.997 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.780 - 1.662 |
| R-factor | 0.237 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dpn |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.029 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.780 | 1.760 |
| High resolution limit [Å] | 1.662 | 1.662 |
| Rmerge | 0.025 | 0.202 |
| Number of reflections | 7781 | |
| <I/σ(I)> | 28.66 | 6.93 |
| Completeness [%] | 99.4 | 99 |
| Redundancy | 3.61 | 3.71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.04M sodium cacodylate buffer, 0.08M potassium chloride, 0.012M sodium chloride, 0.012M spermine tetra hydrochloride, 37% MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
