4GL4
Crystal structure of oxidized S-nitrosoglutathione reductase from Arabidopsis thalina, complex with NADH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-18 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 91.634, 91.634, 173.184 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.130 - 1.800 |
| R-factor | 0.19834 |
| Rwork | 0.196 |
| R-free | 0.23604 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3uko |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.756 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.200 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.072 | 0.390 |
| Number of reflections | 93100 | |
| <I/σ(I)> | 10.8 | 3.2 |
| Completeness [%] | 100 | |
| Redundancy | 11.4 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 2.1 M ammonium sulfate, 100 mM Hepes pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
