4GL1
Structure of H64A/N62L/N67L Human Carbonic Anhydrase II triple mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-07-15 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.070, 41.143, 71.685 |
Unit cell angles | 90.00, 104.08, 90.00 |
Refinement procedure
Resolution | 23.765 - 1.500 |
R-factor | 0.1707 |
Rwork | 0.169 |
R-free | 0.19350 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.195 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.500 |
Number of reflections | 37373 |
Completeness [%] | 97.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 1.0 to 1.3 Sodium Citrate and 50 to 100 mM Tris Buffer pH 7-9, VAPOR DIFFUSION, HANGING DROP, temperature 298K |