4GKZ
HA1.7, a MHC class II restricted TCR specific for haemagglutinin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.91730 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.240, 49.660, 72.610 |
| Unit cell angles | 90.00, 94.33, 90.00 |
Refinement procedure
| Resolution | 26.360 - 2.390 |
| R-factor | 0.25127 |
| Rwork | 0.249 |
| R-free | 0.29812 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fyt |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.745 |
| Data reduction software | xia2 |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 26.364 | 26.364 | 2.450 |
| High resolution limit [Å] | 2.390 | 10.690 | 2.390 |
| Rmerge | 0.014 | 0.576 | |
| Number of reflections | 19445 | ||
| <I/σ(I)> | 18.9 | 41.1 | 1.3 |
| Completeness [%] | 98.4 | 93.6 | 99.6 |
| Redundancy | 3.8 | 3.6 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM Bis-Tris Propane, 200mM Sodium Bromide, 20% PEG 3350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
