4GKR

Structure of the C-terminal motor domain of Kar3 from Candida glabrata

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-06
Detector	ADSC QUANTUM 210
Wavelength(s)	0.917
Spacegroup name	P 21 21 21
Unit cell lengths	58.707, 84.066, 151.127
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.690
R-factor	0.2128
Rwork	0.210
R-free	0.25680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kar
RMSD bond length	0.009
RMSD bond angle	1.359
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.800
High resolution limit [Å]	2.690	5.810	2.690
Rmerge	0.076	0.052	0.639
Number of reflections	20662
<I/σ(I)>	18.2
Completeness [%]	96.2	97.4	91.2
Redundancy	11.7	13	10.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	0.1M MIB buffer, 25% PEG1500, 1mM TCEP, 1mM ATP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K