4GK3
Human EphA3 Kinase domain in complex with ligand 87
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-03-30 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.297, 38.250, 76.050 |
| Unit cell angles | 90.00, 101.72, 90.00 |
Refinement procedure
| Resolution | 30.851 - 1.898 |
| R-factor | 0.1796 |
| Rwork | 0.178 |
| R-free | 0.21280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gsf |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.377 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.020 | 2.000 |
| High resolution limit [Å] | 1.898 | 1.900 |
| Rmerge | 0.084 | 0.405 |
| Number of reflections | 23747 | |
| <I/σ(I)> | 8.9 | 2.8 |
| Completeness [%] | 98.5 | 94.8 |
| Redundancy | 3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M sodium cacodylate pH 6.5, 0.15M ammonium sulf ate, 22.5% PEG 3350 , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
