4GK2
Human EphA3 Kinase domain in complex with ligand 66
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-03-07 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.601, 38.195, 75.704 |
| Unit cell angles | 90.00, 101.71, 90.00 |
Refinement procedure
| Resolution | 33.953 - 2.195 |
| R-factor | 0.1929 |
| Rwork | 0.191 |
| R-free | 0.23610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gsf |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.503 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.190 | 2.310 |
| High resolution limit [Å] | 2.195 | 2.200 |
| Rmerge | 0.103 | 0.457 |
| Number of reflections | 15076 | |
| <I/σ(I)> | 8.8 | 3.5 |
| Completeness [%] | 98.4 | 94.6 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M sodium cacodylate pH 6.5, 0.15M ammonium sulf ate, 22.5% PEG 3350 , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
