4GJ1
Crystal structure of 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.340, 75.733, 45.923 |
| Unit cell angles | 90.00, 105.18, 90.00 |
Refinement procedure
| Resolution | 34.108 - 2.152 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.22900 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.861 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | Auto-Rickshaw |
| Refinement software | PHENIX ((phenix.refine: dev_1117)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.101 | |
| Number of reflections | 12596 | |
| Completeness [%] | 99.7 | 98.6 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 25% PEG 3350 , 0.2 M lithium Sulfate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
