4GIX
Crystal structure of human GLTP bound with 12:0 disulfatide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-09-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 74.768, 50.163, 65.850 |
| Unit cell angles | 90.00, 118.82, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.800 |
| R-factor | 0.18663 |
| Rwork | 0.183 |
| R-free | 0.25053 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rzn |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.383 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 19652 | |
| Completeness [%] | 97.7 | 96.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 15%PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
