4GIT

Crystal structure of alpha sub-domain of Lon protease from Brevibacillus thermoruber

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13C1
Synchrotron site	NSRRC
Beamline	BL13C1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-19
Detector	RAYONIX MX300HE
Wavelength(s)	0.975
Spacegroup name	P 2 3
Unit cell lengths	94.283, 94.283, 94.283
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.815 - 2.882
R-factor	0.2061
Rwork	0.201
R-free	0.24840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3m6a
RMSD bond length	0.009
RMSD bond angle	1.317
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.880	2.880
Rmerge		0.066
Number of reflections	6542
<I/σ(I)>		38
Redundancy		20.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.6	283	0.1M potassium phosphate, 0.2M sodium chloride, 12.5% PEG 8000, pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 283K