4GI0
Crystal structure of CD23 lectin domain mutant E249A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-01-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9763 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 63.320, 63.320, 261.420 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 65.360 - 2.270 |
R-factor | 0.2075 |
Rwork | 0.205 |
R-free | 0.25100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2h2r |
RMSD bond length | 0.010 |
RMSD bond angle | 1.375 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | PHASES |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.400 | 2.400 |
High resolution limit [Å] | 2.270 | 2.270 |
Number of reflections | 25713 | |
Completeness [%] | 100.0 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.2M potassium thiocyanate, 0.1M bis tris propane pH 8.5, 20% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 291K |