4GHP
Crystal Structure of D48V||A47D mutant of Human GLTP bound with 12:0 monosulfatide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9754 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.331, 49.118, 68.120 |
| Unit cell angles | 90.00, 123.58, 90.00 |
Refinement procedure
| Resolution | 14.990 - 1.900 |
| R-factor | 0.21447 |
| Rwork | 0.213 |
| R-free | 0.24627 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ric |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.596 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.850 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Number of reflections | 16808 | |
| Completeness [%] | 98.5 | 96.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 10-20% PEG 3350, pH 5.5-7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
