4GHO
Crystal Structure Analysis of Streptomyces aureofaciens Ribonuclease S24A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-06-13 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.834 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.918, 64.693, 78.281 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.520 - 1.100 |
| R-factor | 0.09793 |
| Rwork | 0.097 |
| R-free | 0.11701 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rgg |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.140 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.110 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Number of reflections | 80959 | |
| <I/σ(I)> | 3.3 | |
| Completeness [%] | 98.2 | 96.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | 0.7M ammonium sulfate, phosphate buffer, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298, temperature 298.0K |
