4GHK
X-ray Crystal Structure of Gamma-glutamyl phosphate reductase from Burkholderia thailandensis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-20 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 85.410, 142.460, 154.760 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.398 - 2.250 |
R-factor | 0.1828 |
Rwork | 0.180 |
R-free | 0.22450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ola |
RMSD bond length | 0.007 |
RMSD bond angle | 1.039 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.310 | |
High resolution limit [Å] | 2.250 | 10.060 | 2.250 |
Rmerge | 0.093 | 0.023 | 0.551 |
Number of reflections | 90052 | ||
<I/σ(I)> | 13.59 | 45.03 | 2.65 |
Completeness [%] | 99.8 | 96 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 40% PEG 300, 200 mM Calcium Acetate, 100 mM sodium cacodylate pH 6.50, VAPOR DIFFUSION, SITTING DROP, temperature 289K |