4GGN
Malaria invasion machinery protein complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97939 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 82.560, 83.403, 84.316 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.700 - 2.290 |
| R-factor | 0.22836 |
| Rwork | 0.226 |
| R-free | 0.27942 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.184 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.700 | 2.340 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.083 | |
| Number of reflections | 26831 | |
| <I/σ(I)> | 23.5 | |
| Completeness [%] | 99.3 | |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1M HEPES, 10% PEG6000, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
