4GGA
Structural Analysis of Human Cdc20 Supports Multi-site Degron Recognition by APC/C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97937 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.865, 87.258, 110.954 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.347 - 2.044 |
| R-factor | 0.1536 |
| Rwork | 0.151 |
| R-free | 0.20300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.327 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1062) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.589 | 2.090 |
| High resolution limit [Å] | 2.044 | 2.050 |
| Rmerge | 0.222 | 0.599 |
| Number of reflections | 24684 | |
| <I/σ(I)> | 10 | |
| Completeness [%] | 95.4 | 83.8 |
| Redundancy | 5.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 100 mM Na citrate, 15 % (w/v) PEG 6000, and 4% MPD, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
