4GG4

Crystal structure of the TAL effector dHax3 bound to specific DNA-RNA hybrid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00915
Spacegroup name	P 61
Unit cell lengths	99.735, 99.735, 134.494
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.725 - 2.501
R-factor	0.1954
Rwork	0.193
R-free	0.24260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3v6t
RMSD bond length	0.007
RMSD bond angle	1.182
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	25803
Completeness [%]	98.4	99.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	291	11% PEG 3350 (w/v), 12% ethanol, 0.1M MES pH6.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K