4GFT
Malaria invasion machinery protein-Nanobody complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.848, 52.951, 73.226 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.530 - 1.600 |
| R-factor | 0.18239 |
| Rwork | 0.181 |
| R-free | 0.20466 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.567 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.600 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.083 | 0.690 |
| Number of reflections | 23569 | |
| <I/σ(I)> | 9 | 1.8 |
| Completeness [%] | 94.8 | 87.3 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 293 | 25% PEG3350, 50 mM ammonium sulfate, 0.1 M TrisHCl, pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
