4GFQ

2.65 Angstrom Resolution Crystal Structure of Ribosome Recycling Factor (frr) from Bacillus anthracis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-04
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.07806
Spacegroup name	P 2 3
Unit cell lengths	95.548, 95.548, 95.548
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.810 - 2.650
R-factor	0.2051
Rwork	0.203
R-free	0.25452
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ek8
RMSD bond length	0.012
RMSD bond angle	1.412
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	BALBES
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.700
High resolution limit [Å]	2.650	2.650
Rmerge	0.061	0.553
Number of reflections	8662
<I/σ(I)>	18	2.5
Completeness [%]	99.3	100
Redundancy	4.3	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	protein: 7.4 mg/mL in 0.25 M sodium cloride, 0.01 M Tris-HCl, pH 8.3, screen: Classics II (H3), 0.24 M sodium malonate, pH 7.0, 20% w/v PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 295K