4GFC

N-terminal coiled-coil dimer of C.elegans SAS-6, crystal form B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Collection date	2010-09-24
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	103.520, 50.720, 101.870
Unit cell angles	90.00, 97.91, 90.00

Refinement procedure

Resolution	51.270 - 2.850
R-factor	0.2155
Rwork	0.214
R-free	0.23620
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.150
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.270	3.000
High resolution limit [Å]	2.850	2.850
Number of reflections	12487
Completeness [%]	100.0	100
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	10% PEG 20,000, 20% PEG MME 550, 0.1M MES/Imidazole buffer pH 6.5 and 0.02M of each of these monosaccharides (D-glucose, D-mannose, D-galactose, L-fucose, D-xylose, N-acetyl-D-glucosamine), VAPOR DIFFUSION, SITTING DROP, temperature 277K