4GFA
N-terminal coiled-coil dimer of C.elegans SAS-6, crystal form A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 73.150, 118.940, 121.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.310 - 3.550 |
| R-factor | 0.3122 |
| Rwork | 0.310 |
| R-free | 0.35740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.180 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.120 | 3.740 |
| High resolution limit [Å] | 3.550 | 3.550 |
| Number of reflections | 13307 | |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 0.2M LiSO4, 0.1M MES pH6, 35% MPD, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
