4GF8

Crystal Structure of the Chitin Oligasaccharide Binding Protein

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Detector	ENRAF-NONIUS
Spacegroup name	P 32 2 1
Unit cell lengths	92.791, 92.791, 136.848
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.300
Rwork	0.198
R-free	0.21970
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.394
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.081	0.211
Number of reflections	30875
<I/σ(I)>	25.9	9.4
Completeness [%]	100.0	100
Redundancy	15.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	ammonium sulfate, PEG400, HEPES, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K