4GF2
Crystal structure of Plasmodium falciparum Erythrocyte Binding Antigen 140 (PfEBA-140/BAEBL)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 2010-10-07 |
| Detector | NOIR-1 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.420, 76.480, 82.340 |
| Unit cell angles | 90.00, 96.80, 90.00 |
Refinement procedure
| Resolution | 19.747 - 2.400 |
| R-factor | 0.2022 |
| Rwork | 0.200 |
| R-free | 0.23690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zro |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.607 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | BALBES |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 31532 | |
| Completeness [%] | 99.4 | 98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 20% PEG 8000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
