4GET
Crystal structure of biogenic amine binding protein from Rhodnius prolixus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.727, 72.331, 79.491 |
| Unit cell angles | 90.00, 90.04, 90.00 |
Refinement procedure
| Resolution | 32.919 - 2.240 |
| R-factor | 0.188 |
| Rwork | 0.185 |
| R-free | 0.25440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.919 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.330 |
| High resolution limit [Å] | 2.240 | 4.850 | 2.240 |
| Rmerge | 0.081 | 0.052 | 0.363 |
| Number of reflections | 37060 | ||
| <I/σ(I)> | 9.1 | ||
| Completeness [%] | 99.2 | 99.8 | 94.1 |
| Redundancy | 5.6 | 5.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.2 | 298 | 28-30% PEG 6000, 0.1 M Tris, pH 8.2, VAPOR DIFFUSION, temperature 298K |
