4GER
Crystal structure of Gentlyase, the neutral metalloprotease of Paenibacillus polymyxa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Detector technology | PIXEL |
| Collection date | 2011-08-23 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.014, 77.070, 64.052 |
| Unit cell angles | 90.00, 103.16, 90.00 |
Refinement procedure
| Resolution | 48.940 - 1.590 |
| R-factor | 0.20184 |
| Rwork | 0.200 |
| R-free | 0.24279 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.052 |
| Refinement software | REFMAC (5.6.0119) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.940 |
| High resolution limit [Å] | 1.590 |
| Number of reflections | 72131 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 277 | 0.2 M magnesium chloride, 0.1 M HEPES, 25% PEG3350, pH 7.5, VAPOR DIFFUSION, temperature 277K |
