4GEN

Crystal structure of Zucchini (monomer)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Collection date	2011-12-07
Wavelength(s)	0.9793, 0.9800
Spacegroup name	P 1 21 1
Unit cell lengths	35.277, 52.396, 38.791
Unit cell angles	90.00, 104.94, 90.00

Refinement procedure

Resolution	37.480 - 2.200
R-factor	0.225
Rwork	0.223
R-free	0.26990
Structure solution method	MAD
RMSD bond length	0.002
RMSD bond angle	0.566
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.240
High resolution limit [Å]	2.200	5.970	2.200
Rmerge	0.097	0.061	0.354
Number of reflections	6839
<I/σ(I)>	10.8
Completeness [%]	97.1	98.4	94.6
Redundancy	5.4	6.9	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION			vapor diffusion