4GE3
Schizosaccharomyces pombe DJ-1 T114V mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-07-12 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.579, 52.075, 82.912 |
| Unit cell angles | 89.01, 88.98, 66.55 |
Refinement procedure
| Resolution | 82.890 - 1.500 |
| R-factor | 0.15992 |
| Rwork | 0.158 |
| R-free | 0.18864 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4GDH |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.504 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.072 | 0.253 |
| Number of reflections | 102613 | |
| <I/σ(I)> | 16.6 | 5.9 |
| Completeness [%] | 93.4 | 87.6 |
| Redundancy | 5.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 26% PEG4000, 100 mM Tris-HCl, 200 mM magnesium chloride, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
