4GE1
Structure of the tryptamine complex of the amine binding protein of Rhodnius prolixus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.92309 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.257, 73.086, 80.935 |
| Unit cell angles | 90.00, 91.45, 90.00 |
Refinement procedure
| Resolution | 25.348 - 2.150 |
| R-factor | 0.195 |
| Rwork | 0.192 |
| R-free | 0.24930 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.903 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | FFT |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.190 |
| High resolution limit [Å] | 2.150 | 5.830 | 2.150 |
| Rmerge | 0.076 | 0.037 | 0.615 |
| Number of reflections | 43654 | ||
| <I/σ(I)> | 11.3 | ||
| Completeness [%] | 99.0 | 98.2 | 91.8 |
| Redundancy | 5.2 | 5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.2 | 298 | 28-30% PEG 6000, 0.1 M Tris, pH 8.2, VAPOR DIFFUSION, temperature 298K |
