4GDP
Yeast polyamine oxidase FMS1, N195A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-04-24 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 1 |
| Unit cell lengths | 78.851, 81.885, 104.791 |
| Unit cell angles | 78.07, 79.51, 78.92 |
Refinement procedure
| Resolution | 79.074 - 2.000 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.23390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ech |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.875 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 149689 | |
| <I/σ(I)> | 10.5 | 3.2 |
| Completeness [%] | 89.0 | 75.3 |
| Redundancy | 3.4 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 0.12 M Di-Ethyleneglycol, Tri-Ethyleneglycol, Tetra-Ethyleneglycol, Penta-Ethyleneglycol, 0.1M Imidazole; MES, 30% Ethylene glycol, PEG 8000 , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
