4GDN
Structure of FmtA-like protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 179.710, 179.710, 287.501 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 85.730 - 3.200 |
| R-factor | 0.25921 |
| Rwork | 0.257 |
| R-free | 0.29503 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o3v |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.587 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.760 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.100 | 0.360 |
| Number of reflections | 42000 | |
| <I/σ(I)> | 8.2 | 3.2 |
| Completeness [%] | 97.3 | 98.4 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.2M ammonium acetate, 0.1M sodium acetate trihydrate, 30% W/v polyethylene glycol 4000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
