4GDJ

A subtype N10 neuraminidase-like protein of A/little yellow-shouldered bat/Guatemala/060/2010

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-05
Detector	MARMOSAIC 300 mm CCD
Spacegroup name	P 41 21 2
Unit cell lengths	107.902, 107.902, 345.210
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.850 - 2.000
R-factor	0.191
Rwork	0.189
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.579
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	2.000	2.000
Number of reflections	135663
<I/σ(I)>	18.8	1.7
Completeness [%]	98.0	86.8
Redundancy	3.9	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8	295	0.2 M Mg nitrate, 20% glycerol, 18% polyethylene glycol 4000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K