4GDI

A subtype N10 neuraminidase-like protein of A/little yellow-shouldered bat/Guatemala/164/2009

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-22
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	I 4
Unit cell lengths	176.324, 176.324, 193.313
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.110 - 1.950
R-factor	0.182
Rwork	0.180
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3beq
RMSD bond length	0.009
RMSD bond angle	1.161
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.980
High resolution limit [Å]	1.950	1.950
Number of reflections	210731
<I/σ(I)>	23.1	1.5
Completeness [%]	98.8	99.5
Redundancy	2.6	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	295	0.2 M Mg nitrate, 14% polyethylene glycol 3350, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K