4GCZ
Structure of a blue-light photoreceptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-18 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 105.216, 105.216, 441.958 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.499 - 2.300 |
| R-factor | 0.1806 |
| Rwork | 0.179 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.141 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.440 |
| High resolution limit [Å] | 2.300 | 6.840 | 2.300 |
| Rmerge | 0.074 | 0.026 | 0.499 |
| Number of reflections | 121332 | 4637 | 19429 |
| <I/σ(I)> | 18.67 | 48.39 | 3.73 |
| Completeness [%] | 99.8 | 99.7 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | KF, (NH4)2SO4, pH 8.0, vapor diffusion, sitting drop, temperature 291K |
