4GCY
Structure of Mycobacterium tuberculosis dUTPase H21W mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-09-06 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 63 |
| Unit cell lengths | 54.940, 54.940, 83.860 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.470 - 1.500 |
| R-factor | 0.12492 |
| Rwork | 0.123 |
| R-free | 0.15830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.555 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.950 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.085 | 0.578 |
| Number of reflections | 23022 | |
| <I/σ(I)> | 11.8 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.5 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 1.5M ammonium sulphate, 0.1M Tris/HCl, 10% glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
