4GCO

Central domain of stress-induced protein-1 (STI-1) from C.elegans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	32.959, 50.570, 72.112
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.400 - 1.600
R-factor	0.1793
Rwork	0.177
R-free	0.21880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1elw
RMSD bond length	0.017
RMSD bond angle	1.667
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.400	50.000	1.630
High resolution limit [Å]	1.600	4.340	1.600
Rmerge	0.052	0.032	0.355
Number of reflections	16343
<I/σ(I)>	12.5		2.02
Completeness [%]	98.8	96.8	84
Redundancy	8.4	8	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	0.1 M Bis-Tris buffer, 25% PEG-3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K