4GC3

Crystal structure of L-HISTIDINOL PHOSPHATE PHOSPHATASE (HISK) from Lactococcus lactis subsp. lactis Il1403 complexed with ZN and sulfate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.075
Spacegroup name	P 21 21 21
Unit cell lengths	51.480, 76.495, 78.057
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.028 - 1.320
R-factor	0.1523
Rwork	0.152
R-free	0.16200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.088
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	39.028
High resolution limit [Å]	1.320
Number of reflections	72355
Completeness [%]	99.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	25% PEG 4000, 0.1M sodium acetate, 0.2M ammonium sulfate, 0.5 mM Zinc Chloride, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K