4GBZ

The structure of the MFS (major facilitator superfamily) proton:xylose symporter XylE bound to D-glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-02-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9789
Spacegroup name	P 43 21 2
Unit cell lengths	95.036, 95.036, 168.566
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.524 - 2.894
R-factor	0.2489
Rwork	0.247
R-free	0.28880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.207
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.000
High resolution limit [Å]	2.890	2.900
Number of reflections	17572
Completeness [%]	98.1	97.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.6	291	40 % (w/v) PEG400, 0.05 M Glycine pH 9.6 and 0.1 M LiCl , VAPOR DIFFUSION, HANGING DROP, temperature 291K