4GAL

CRYSTAL STRUCTURE OF HUMAN GALECTIN-7 IN COMPLEX WITH LACTOSE

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.5
Synchrotron site	SRS
Beamline	PX9.5
Temperature [K]	289
Detector technology	IMAGE PLATE
Collection date	1997-08
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	54.290, 65.410, 73.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.950
R-factor	0.2
Rwork	0.200
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	FREE GALECTIN-7
RMSD bond length	0.010
RMSD bond angle	30.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.050
High resolution limit [Å]	1.950	1.950
Rmerge	0.047
Total number of observations	80604 *
Number of reflections	19246
<I/σ(I)>	7.6	3.9
Completeness [%]	96.8	92.4
Redundancy	4.2	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.1	16 *	CRYSTALS WERE GROWN USING THE HANGING DROP METHOD FROM DROPS CONTAINING 9 MG/ML PROTEIN AT PH 8.1 IN 50 MM SODIUM PHOSPHATE BUFFER, 0.3 M SODIUM CHLORIDE, 20 MM IMIDAZOLE, 8.5% PEG 3350. DROPS WERE EQUILIBRATED AGAINST RESERVOIRS CONTAINING 50 MM SODIUM PHOSPHATE BUFFER, 0.3 M SODIUM CHLORIDE, 20 MM IMIDAZOLE, 17% PEG 3350. GALECTIN-7 CRYSTALS WERE THEN SOAKED WITH 5 MM LACTOSE FOR 2 HRS., vapor diffusion - hanging drop

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	9 (mg/ml)
2	1	drop	sodium phosphate	50 (mM)
3	1	drop		0.3 (M)
4	1	drop	imidazol	20 (mM)
5	1	drop	PEG3350	8.5 (%)
6	1	reservoir	sodium phosphate	50 (mM)
7	1	reservoir		0.3 (M)
8	1	reservoir	imidazole	20 (mM)
9	1	reservoir	PEG3350	17 (%)