4GA9
Crystal Structure of Rat Galectin-1 in Complex with Lactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-01 |
| Wavelength(s) | 0.933 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 106.388, 106.440, 107.978 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.880 |
| R-factor | 0.2183 |
| Rwork | 0.215 |
| R-free | 0.26990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.131 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.780 | 1.929 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Number of reflections | 27931 | |
| Completeness [%] | 98.1 | 98.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | Bis-Tris Propane, lithium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
