4GA4

Crystal structure of AMP phosphorylase N-terminal deletion mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	95
Detector technology	CCD
Collection date	2010-10-12
Detector	ADSC QUANTUM 210
Wavelength(s)	1.000
Spacegroup name	P 65
Unit cell lengths	166.122, 166.122, 93.439
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 3.510
Rwork	0.272
R-free	0.32200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dsj
RMSD bond length	0.013
RMSD bond angle	1.700
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.640
High resolution limit [Å]	3.510	3.510
Number of reflections	18550
<I/σ(I)>		5.9
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	0.1M sodium citrate tribasic dihydrate, 1.65M 1,6-hexanediol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K