4GA2
Structure of the N-terminal domain of Nup358
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-01-17 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.7500 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 58.788, 83.166, 29.559 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.900 - 0.950 |
| R-factor | 0.1778 |
| Rwork | 0.177 |
| R-free | 0.19890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.311 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.900 | 0.980 |
| High resolution limit [Å] | 0.950 | 0.950 |
| Number of reflections | 91604 | |
| <I/σ(I)> | 18 | 1.6 |
| Completeness [%] | 99.6 | 98.7 |
| Redundancy | 12.2 | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 18 % (w/v) PEG 3350 200 mM lithium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
