4G9S

Crystal structure of Escherichia coli PliG in complex with Atlantic salmon g-type lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-02-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.8, 1.8233
Spacegroup name	P 65
Unit cell lengths	132.182, 132.182, 42.902
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.267 - 0.950
R-factor	0.1294
Rwork	0.129
R-free	0.13630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3MGW AND 4DY3
RMSD bond length	0.006
RMSD bond angle	1.149
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.430	1.000
High resolution limit [Å]	0.950	0.950
Number of reflections	265542
<I/σ(I)>		0.7
Completeness [%]	98.5	92.4
Redundancy		3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	1.8M tri-ammonium citrate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7	293	1.8M tri-ammonium citrate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K