4G9K

Structure of the Ndi1 protein from Saccharomyces cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-01-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	116.120, 165.560, 70.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.320 - 2.700
R-factor	0.2354
Rwork	0.234
R-free	0.26770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.959
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.700
Number of reflections	35714
Completeness [%]	96.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	34% PEG 400, 100 mM NaCl, 2% ethylene glycol, 5% glycerol, 50 mM MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K