4G9I
Crystal structure of T.kodakarensis HypF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-18 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97600 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 265.810, 265.810, 693.712 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 4.500 |
| R-factor | 0.279 |
| Rwork | 0.279 |
| R-free | 0.30000 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.660 |
| High resolution limit [Å] | 4.500 | 4.500 |
| Number of reflections | 143223 | |
| <I/σ(I)> | 12.6 | 3.4 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 9.3 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 293 | 0.1M Tris-HCl, 3.7M sodium chloride, pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
