4G9B
Crystal structure of beta-phosphoglucomutase homolog from escherichia coli, target efi-501172, with bound mg, open lid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-02-03 |
Detector | RAYONIX 225 HE |
Wavelength(s) | 0.9793 |
Spacegroup name | H 3 2 |
Unit cell lengths | 129.168, 129.168, 85.766 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.588 - 1.700 |
R-factor | 0.1673 |
Rwork | 0.166 |
R-free | 0.20380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3nas |
RMSD bond length | 0.007 |
RMSD bond angle | 1.019 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.16) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 68.063 | 26.588 | 1.790 |
High resolution limit [Å] | 1.700 | 5.380 | 1.700 |
Rmerge | 0.108 | 0.041 | 0.875 |
Total number of observations | 9715 | 48098 | |
Number of reflections | 30181 | ||
<I/σ(I)> | 12.6 | 16.5 | 0.9 |
Completeness [%] | 99.9 | 96 | 100 |
Redundancy | 11.1 | 10 | 11 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | sitting drop vapor diffuction | 7 | 298 | Protein (10 mM Hepes pH 7.5, 150 mM NaCl, 10% glycerol, 1 mM DTT, 5 mM MgCl); Reservoir (0.1 M Bis-Tris Propane:HCl, 1.8 M Magnesium Sulfate); Cryoprotection (Reservoir, + 20% glycerol), sitting drop vapor diffuction, temperature 298K |