4G98

Rat Heme Oxygenase-1 in complex with Heme and CO at 100K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-21
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 32 2 1
Unit cell lengths	65.974, 65.974, 120.018
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.990 - 2.300
R-factor	0.15654
Rwork	0.153
R-free	0.21767
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1ix4
RMSD bond length	0.017
RMSD bond angle	2.862
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC (5.6.0117)
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Number of reflections	14009
<I/σ(I)>	13.2	6.2
Completeness [%]	99.8	100
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	4M sodium formate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K