4G92
CCAAT-binding complex from Aspergillus nidulans with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-01-28 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.040, 72.890, 145.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| R-factor | 0.15843 |
| Rwork | 0.157 |
| R-free | 0.19008 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4g91 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.093 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0119) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.059 | 0.395 |
| Number of reflections | 54543 | |
| <I/σ(I)> | 11.7 | 2.5 |
| Completeness [%] | 95.3 | 96.8 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES, 0.2 M (NH4)2SO4, 25% PEG3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
