4G91
CCAAT-binding complex from Aspergillus nidulans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-08-02 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.450, 60.700, 71.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.18059 |
| Rwork | 0.179 |
| R-free | 0.20223 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n1j |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.964 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0119) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.057 | 0.332 |
| Number of reflections | 20415 | |
| <I/σ(I)> | 24.4 | 6.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1 M Tris, 20% PEG 6000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
