4G90
Crystal structure of Ribonuclease A in complex with 5e
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-22 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.399, 32.631, 72.461 |
| Unit cell angles | 90.00, 90.95, 90.00 |
Refinement procedure
| Resolution | 13.861 - 1.900 |
| R-factor | 0.1778 |
| Rwork | 0.175 |
| R-free | 0.23580 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2g8q |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.192 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 18814 | |
| <I/σ(I)> | 12.7 | 2.8 |
| Completeness [%] | 99.6 | 98.9 |
| Redundancy | 3.84 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 25% PEG 4000, 20 mM Nacitrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
